Tuesday, December 15, 2009

fyp-devotion for a scroll

my project entitled "study of wound healing effects of Channa sp. on fibroblast cells"

what is Channa sp.? Its a species of freshwater fish commonly found in most asian freshwaters region. Snakehead is their native name..But, to be precise, in Malaysia, it is known as "ikan haruan" (since there are many any kinds of snakehead in here such as toman and bujuk)...

Bet you guys know this product-"polleney's extract of haruan@pati ikan haruan polleney"...well...., my project has nothing to do with it...hehe

more or less, my project begins with catching the fish that lives wild(supposedly =P), get the fillet of its muscle (the flesh that we eat), and proceed with the "hard-to-do" extraction procedure in order to obtain 2 kinds of extract- crude fats and proteins..(done with the fat...alhamdulillah~)

Next, biochem analysis; gcms for the fat contents and spectro&SDS-PAGE for the total proteins...(currently trying to pass this stage -_- )

At the same timt, im working on sub-culturing the HaCaT cells..(it supposed to be fibroblast but my supervisor changed it to HaCaT...just follow without hesitation....well, he's the boss,right??..hehe)
to be honest, this stage is fun and interesting...
after some times, i will scratch the cells (this action represent the wound formation)... (T_T-the cell's crying...its painful!!)

ok, next..!!! the extract will be applied onto the cell culture...
and i'll observe and measure the cell's proliferation activity...
and compare this with the control (without extract)..
by assuming that the extract is having the potential for wound healing, the cells with the extract should be proliferate faster than those who are not...

i think, that's all for the project...i'm still in the first-entering second stage out of TEN!!! there's far way more to go...~~~

since this started, my whole life-mind and soul, is all about ikan haruan.....huuu~ n_n

Sunday, December 13, 2009

L.I.F.E- "life" is "finding experiences"~

i awiz wonder everything that happens to me and people around me..
most people said there's awiz reason behind every single thing..

experiment= observation+analysis --> result+conclusion
life is just like an experiment...
for us to see...to observe...with purpose...
i like to observe thing/people....
those observations make me think (analysis)...
n come out with a rsult...(which i cnsider as experience)
that usually will reflect in my decision making process..

but an equipment does not awiz well-functioning...
lead to an error..resulting in false +ve/-ve result...
considering the equipment is "me"..(who is always with "mulfunction") =P
dats da reason why sometimes (OR most o da tym),
i failed to make wise judgement before my action...

To reduce da likelihood of error, a machine shud be calibrated...
strengthening da "iman" is da best way of duin it...
but it is not easy to do than said..

Yet, whenever it is said "not easy", it doesn't mean its impossible/hard to b done..
there are so many ways...
literally, it is not just about praying, zikr, etc...
there r so many other ways...

For everything that happens, be it good or bad, there must be something that Allah wants us to know, to learn...belief dat many of us already knew bout dz...
but do we really have "faith" in it??..

to be continued.... =P

testing 1 2 3..

thanx allah ta'ala kerana berjaya juga menyiapkan blog ini untuk orang yang saya sayangi..may allah bless her..good luck in using this ordinary blog..muah :)